Group One Prince of Wales Stakes was supposed to be Godolphin owned-
Masar's first run back but trainer Appleby has confirmed he will run in the Hardwicke Stakes at Royal Ascot instead.
Appleby explained he and the owners on last year's Epsom Derby winner after the colt galloped on Saturday.
The decision also means James Doyle will be free to ride William Haggas' Sea Of Class in the Prince of Wales's, rather than having to choose Masar in that race as he continues to deputise for his fellow Godolphin rider William Buick - who is currently absent with illness.